Analysis of menthol obtained from the extraction of menthol from the formulation was done by the proposed method. Identification and determination were performed by BP-1 Column (30.0 m × 0.32 mm ID) using nitrogen at a flow rate of 3.0 ml/min using FID detector. The developed conditions for GC were column oven temperature (75°C-15 min-@40°C/min-170°C-3 min), total run time (20 min), injection temperature (220°C), detector temperature (260°C) and sample volume for injection was 1 μl. The retention time for menthol was found to be 13.64 min. The linearity range was found to be 1250-6250 µg/ml for menthol. The LOD and LOQ value for menthol was found to be 105.87 µg/ml and 320.83 µg/ml. The method was validated for linearity, accuracy, precision and specificity.
Loading....